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4.8.00.01: BMC PATROL Knowledge Module for Microsoft Windows Operating System. The BMC PATROL for Microsoft Windows 4.8.00.01 patch corrects issues reported in earlier versions of BMC PATROL for letter writing, Microsoft Windows. This patch modifies the behaviour of Windows Services KM as follows: All Trigger Start services will be configured to monitor without generating an alert. Hence, if the Automatic (Trigger Start) service is book report not running, an alarm or warning will not be generated. Also, the service will not be restarted by the KM. You can change the writing for high students monitor without alert configuration of the Automatic (Trigger Start) service to persuasive pro choice monitor with alert by using the Configure Service KM command. If AutoResetServiceConfig pconfig variable is set to 1 then a ny change in the trigger information will reset the service configuration depending on school the new Trigger information. When you configure Services monitoring by using CMA policy, all the services including the Trigger Start services will have the configuration as defined in the policy configuration. In remote monitoring, Automatic (Trigger Start) services will have the same configuration as that of the Automatic services. Hence, if the Automatic (Trigger Start) service is not running, an alarm will be generated. This patch will reset configuration of existing Automatic (Trigger Start) services to monitor without generating an alert.
This topic provides instructions for downloading and installing the patch. Downloading the patch and installation utility. This section provides the information that you need to download and install the patch from the BMC Electronic Product Distribution (EPD) website. To download the patch and installation utility from the EPD website, perform the following steps: Create a directory in which to place the downloaded files. On Windows computers, ensure that the directory is only one level into tendulkar as a role model, the directory structure.
The EPD package creates a directory in the temporary directory when you extract the letter writing for high files, and the directory that contains the installation image should not be in a directory deeper than two levels into the directory structure. Othello Book Report! In the letter for high students Filter Products: field, type BMC PATROL Installation Utility . Click Go . In the right pane, click BMC PATROL Installation Utility . You can use BMC PATROL Installation Utility 9.5.00 or later. In the Products tab, based on the operating system, select the check box next to thank you letter application rejection the files. BMC PATROL Installation Utility Version 9.6.00 - Windows Only. This method requires a one-time installation of the letter writing for high school students Akamai NetSession client program on the target computer and is usually the letter to a faster and more reliable way to transfer files. A checksum operation is used to verify file integrity automatically. Click Back to writing for high school students Product List . In the Filter Products: field, type BMC PATROL for Microsoft Windows . Click Go . In the right pane, click BMC PATROL for Microsoft Windows . In the Version: list, select 4.8.00 and click Go . In the Patches tab, select the check box next to the file. This method requires a one-time installation of the Akamai NetSession client program on the target computer and is usually the faster and more reliable way to transfer files. A checksum operation is used to verify file integrity automatically. Complete the installation procedure described in the Installing the patch section.
Click Go To Licensed Products View . Locate BMC Performance Manager for Servers and expand its entries to show the available version numbers. Expand BMC Performance Manager for Servers 3.5.00 to show the available components. Expand BMC PATROL Installation Utility 9.6.01 to show the available files. You can use BMC PATROL Installation Utility 9.5.00 or later. Select the check box next to the files, based on the appropriate platforms.
This method requires a one-time installation of the Akamai NetSession client program on the target computer and is usually the essays by alice walker faster and more reliable way to transfer files. A checksum operation is used to verify file integrity automatically. Click Product Patches tab. In the Product Patches tab, expand BMC Performance Manager for Servers and letter writing school expand its entries to show the available version numbers. Expand BMC Performance Manager for Servers 3.5.00 to show the available components. Expand the BMC PATROL for Microsoft Windows 4.8.00 component to show the available files. Select the check box next to essays the patches. This method requires a one-time installation of the Akamai NetSession client program on writing the target computer and is usually the faster and othello book report more reliable way to letter writing transfer files. A checksum operation is used to you letter application rejection verify file integrity automatically. Complete the installation procedure described in the Installing the patch section. Writing! Before you install the patch, you must have previously installed BMC PATROL for othello, Microsoft Windows 4.8.00.
The patch does not contain all of the writing for high school files that are required for a complete product release. You must install the book report patch on the following computers: Hosts that are running the writing for high school students PATROL Agent Hosts that are running the you letter rejection PATROL Console. Before you install the patch, complete the following steps: Stop the PATROL Agent. Back up the for high school PATROL_CACHE directory. Delete all BMC PATROL for how to an application letter to a school, UNIX and Linux files from the PATROL_CACHE directory. Back up the PATROL_HOME/lib/knowledge and PATROL_HOME/lib/psl directories. To install the BMC PATROL for Microsoft Windows 4.8.00.01 patch. After you have downloaded the installation utility and patch files, perform the following steps to install the letter for high school students patch: Extract the installation utility: From the bmc_products directory, run the installation utility as appropriate for your operating system: For Windows, double-click setup.exe.
Follow the on-screen instructions until the write an application letter to a school Select Products and Components to Install page is displayed. On the Select Products and Components to Install page, select PATROL Knowledge Module for Microsoft Windows Operating System - Patch 4.8.00.01 . Follow the on-screen instructions to complete the installation. After you complete the installation steps, you can delete the temporary directory and its contents.
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Essay: Gene transfer techniques – pros and for high students, cons. Gene transfer is persuasive essays on lowering the drinking a technique to stably and efficiently introduce functional genes (that are usually cloned) into the target cells. Genetic transfer is the mechanism by writing for high school, which DNA is transferred from a donor to thank after a recipient. Once donor DNA is inside the recipient, crossing over can occur. The result is a recombinant cell that has a genome different from either the letter writing, donor or the recipient. A recombination event must occur after transfer in order that the change in essay the genome be heritable (passed on to the next generation).The genes are the blueprints essential to school generate all the proteins in our bodies which eventually perform all the persuasive essays on lowering 18, biological functions. Therefore, when a gene is efficiently introduced into a target cell of the host, the protein which is encoded by that gene is produced . Gene transfer technologies developed initially as a research tool for studying the gene expression and letter writing for high school, its function. A range of techniques and naturally occurring processes are utilized for the gene transfer. Techniques are available for the introduction of read by alice walker DNA into many different cell types in letter school culture, either to study gene function and regulation or to produce large amounts of recombinant protein. Cell cultures have therefore been used on a commercial scale to synthesize products such as antibodies, hormones, growth factors, cytokines and abortion pro choice, viral coat proteins for immunization.The most important application of this technology is in letter writing school students vivo gene therapy, i.e. the introduction of DNA into the cells of live animals in order to treat disease.
The extraordinary potential of altered DNA molecule is to give rise to new life-forms that are better adopted for survival. Essays On Lowering Age To? Change in base sequence of DNA leads to change in protein causing disease condition which can be corrected by manipulation of gene. Writing For High? Much emphasis has been given to the gene manipulation in cardiovascular diseases, parkinson’s disease, lysosomal disorders, ocular gene therapy and osteoarthritis. Overview of gene-transfer strategies: Gene transfer can be achieved essentially via three routes. The most straightforward is direct DNA transfer, the physical introduction of foreign DNA directly into the cell. For example, in cultured cells this can be done by microinjection, whereas for cells in vivo direct transfer is often achieved by bombardment with tiny DNA-coated metal particles. The second route is termed transfection, and this encompasses a number of techniques, some chemical and some physical, which can be used to persuade cells to take up DNA from othello their surroundings.
The third is to package the for high, DNA inside an. animal virus, since viruses have evolved mechanisms to persuasive essays the drinking age to naturally infect cells and introduce their own nucleic acid. The transfer of foreign DNA into a cell by this route is letter students termed transduction. The choice of methods of how to an application school DNA transfer depends upon letter writing school, the target cells in which transformation will be performed. It also depends upon the objectives of gene manipulation. The transfection may be either stable or transient. Although, choice of DNA transfer method is very important, the sachin tendulkar as a role model, other important steps are selection of gene, isolation of gene, preparing recombinant DNA and selection of students transformed cells. The regeneration of organism with new characteristics is also equally important. The delivery of genes in vitro can be done by treating the cells with viruses such as retrovirus or adenovirus, calcium phosphate, liposomes, particle bombardment, fine needle naked DNA injection, electroporation or any combination of persuasive 18 these methods.
These are the letter, powerful tools for research and book report, have possible applications in letter students gene therapy. FACTORS TO CONSIDER WHEN SELECTING A GENE DELIVERY METHOD. The advancement of new technologies has made gene delivery both more efficient yet more confusing given the formidable array of reagents and methods now available. Determining the book, best gene delivery approach for any particular experiment requires careful consideration of school students a number of different factors as stated below. 1. Tendulkar As A Role Essay? Cellular Context or Environment – The context or environment of the cells (i.e., in vivo vs. in. vitro vs. ex vivo) to which nucleic acids will be delivered is the letter writing, first factor to consider when. deciding upon a gene transfer method.
The obstacles and challenges posed by delivery to in. vitro cell cultures are very different than those posed by in vivo or ex vivo cells, and require. different tools and techniques for overcoming them. Othello Book Report? For example, targeting nucleic acids to. particular cells while avoiding other cells is usually a major concern for in vivo gene transfer. applications, sometimes a concern for ex vivo applications, but usually not a concern for letter writing in vitro. Another important issue that must be addressed when planning in model essay vivo (but not in vitro) gene. delivery studies is the letter students, immunogenicity of the gene transfer system. This is particularly important when considering the by alice walker, use of certain viral vectors discussed previously, which may be highly immunogenic.
Also related to immunogenicity is the issue of general safety, both for the experimenter and for the subject of any study utilizing transfection. Almost always, the use of viral vectors requires very extensive safety precautions than does the use of synthetic transfection methods. 2. Cell or Tissue Type – The origin of the cells or tissue to which nucleic acids will be. transferred is typically the second parameter considered when selecting a gene delivery. method. For a number of different reasons, the difficulty of cellular nucleic acid delivery varies. from cell type to cell type. Because of this, it is always important to review the literature to. determine if the cell type of interest has previously been utilized for gene transfer experiments, which exact methods have been used, and what delivery efficiencies were achieved, i.e., what. percentages of writing school students cells actually transfected or transduced, with each method.
Certain cell types are often described as ‘notoriously difficult to transfect’. These include primary cell cultures of many varieties. Successful gene transfer to such cells. usually requires a good deal of rejection research before selecting any particular gene delivery method. 3. Delivery Efficiency – For most applications, transfecting or transducing as many cells as.
possible is preferred, especially when it is necessary to detect the presence of rare transgene. products. Students? Thus, it is tendulkar as a role model essay important to determine the level of writing for high students delivery efficiency required for a given. application before deciding on othello book report a particular gene transfer method. In most cases, it is writing found that.
viral vectors provide the highest delivery efficiencies. For some commonly used and read, easily transfected cell lines, cationic lipids nearly match viral vectors in their gene transfer efficiency, while providing safer and much quicker procedures. 4. Stable vs. Transient Gene Delivery – Gene transfer procedures are frequently categorized. by whether the delivered gene remains separate from the writing school students, host cell chromosome or whether it is integrated into the host cell chromosome. In the first case, known as transient gene delivery, expression of the transgene typically dissipates after a given period of sachin as a time – usually within. several days – because the expression vector is either degraded or expelled from the host cell.
In the second case, known as stable gene delivery, expression of the transferred gene is. prolonged, or stable, because the school students, vector is integrated into the host cell chromosome. Obviously, different applications require different time periods of transgene expression. Essays The Drinking Age To? Thus, a careful. comparison between different gene delivery methodologies will allow a suitable choice for. generating the desired transient or stable expression. In the case of viral vectors, determining whether a particular virus is suited for stable or transient. transduction is students simply a matter of learning the general characteristics of the vector. In the essays by alice, case.
of chemical or physical/mechanical gene transfer, determining whether a given method is. suitable for transient vs. stable delivery may be as easy as reading sales literature from the. manufacturer or checking primary research literature. 5. Type of Delivered Molecule – Another factor to consider when selecting a gene delivery. method is letter for high students what type of nucleic acid or other molecule is being transfected or transduced. For. example, a reagent that efficiently delivers plasmids may not efficiently deliver DNA. oligonucleotides.
It is frequently easiest to simply review the how to write an application letter school, literature to learn what methods have been used successfully, and to contact other researchers who have experience delivering the letter writing school students, molecule of how to write to a school interest. 6. Cytotoxicity – Another issue that comes up frequently when choosing among different gene. delivery methods is the cytotoxicity of the various methods and reagents. For High School? Some methods, such. as electroporation and gene guns, can be extremely harsh on cells and often result in the death. of a majority of cells. Also, the DEAE-dextran can be quite harsh on some cell types, especially. when a DMSO or glycerol shock step is used as part of the procedure. In some applications, such as when a small number of transfectants are required from a recalcitrant cell type, this is.
not a problem. In the majority of applications however, a high percentage of cell death is simply. not acceptable. The plethora of available cationic lipids and polymer reagents have varying. degrees of toxicity with different cell types, and their concentrations during transfection can be. optimized as needed to minimize cell death. 7. Suspension vs. Adherent cells – Most commonly transfected cells grow – and are. transfected – while attached to some type of support, whether that support is the tissue from. which they are derived, some inert membrane or scaffolding, or the surface of a tissue culture.
plate. Less common are cells that grow while suspended in medium, such as those derived from. blood and other bodily fluids. Suspension cells are generally more difficult to transfect; therefore, it may be necessary to rely on harsher gene delivery methods, such as. electroporation, gene guns, microinjection, or viral vectors for acceptable transfection levels. Also, some cationic lipids can effectively transfect suspension cells, and should be tried when.
possible due to their simplicity and cost-effectiveness. 8. Expertise – The degree of difficulty in performing the abortion, various transfection techniques can. vary significantly, and therefore the expertise and experience of the researcher is usually an. important factor to consider. For example, methods like microinjection viral transfection methods are invariably more complex than cationic lipids and polymer reagents. Also, of the letter for high, chemical methods, calcium phosphate co-precipitation can be quite tricky due to its sensitivity to slight changes in experimental conditions. 9. Time – Another important factor to consider is the time required for gene delivery.
Viral. methods, while highly efficient, can be quite time consuming, typically taking anywhere from two weeks to you letter rejection one month to complete. In contrast, chemical and physical/mechanical methods can usually be completed within a few days, but frequently provide lower transfection efficiencies. Thus, it is not uncommon to writing school have to weigh the abortion persuasive essay, benefits of faster results and writing school students, less labor against the benefit of sachin tendulkar role model higher efficiency. 10 Cost – Finally, cost can be a major factor when deciding on the best gene delivery. method for a given application. The most expensive methods tend to be the. physical/mechanical methods, such as electroporation and gene guns, due to the specialized.
equipment they require.Viral gene delivery is letter writing perhaps the second most expensive method, since it requires the to a school, vector reagents, extensive time and labor to letter writing for high school students complete lengthy vector preparation procedures, as well as proper disposal of viral waste materials. The third most expensive gene delivery methods utilize polymers and cationic lipids, which do not require any specialized equipment or lengthy viral vector preparation steps. Such reagents typically cost in the hundreds of dollars, depending on the number of transfections to to a be completed. Finally, the least expensive methods utilize calcium phosphate, which is a cheap and abundant mineral, and naked DNA, which obviously requires no transfection reagent cost, but can be highly inefficient. GENE TRANSFER TECHNIQUES. Transformation is the naturally occurring process of gene transfer which involves absorption of the genetic material by a cell through cell membrane causing the fusion of the writing students, foreign DNA with the native DNA resulting in the genetic expression of the received DNA. Transformation involves the uptake of “naked” DNA (DNA not incorporated into structures such as chromosomes) by competent bacterial cells. Thank Rejection? Cells are only competent (capable of taking up DNA) at a certain stage of their life cycle, apparently prior to the completion of cell wall synthesis . Transformation is usually a natural method of gene transfer but as a result of letter school students technological advancement originated the artificial or induced transformation.
Thus there are two types called as natural transformation and artificial or induced transformation. Essays By Alice Walker? In natural transformation, the foreign DNA attaches itself to letter writing the host cell DNA receptor and with the help of the protein DNA translocase it enters the host cell. The presence of nucleases restricts the entry of two strands of the DNA, destroys a single strand thus allowing only one strand to enter the host cell. Any DNA that is not integrated into the chromosome will be degraded. This single stranded DNA mingles with the host genetic material successfully. The artificial or induced method of transformation is done under laboratory condition which is either a chemical mediated gene transfer or done by after, electroporation..
Genetic engineers are able to induce competency by putting cells in certain solutions, typically containing calcium salts. At the entry site, endonucleases cut the letter writing school, DNA into fragments of 7,000-10,000 nucleotides, and the double-stranded DNA separates into single strands. The single-stranded DNA may recombine with the host’s chromosome once inside the the drinking 18, cell. This recombination replaces the gene in the host with a variant – albeit homologous – gene. DNA from a closely related genus may be acquired but, in general, DNA is letter students not exchanged between distantly related microbes.
Not all bacteria can become competent. Conjugation is a means of rejection gene transfer in many species of bacteria. Cell-to-cell contact by a specialized appendage, known as the F-pilus (or sex pilus), allows a copy of an F- plasmid (fertility plasmid) to transfer to a cell that does not contain the plasmid. On rare occasions an for high students F-plasmid may become integrated in the chromosome of tendulkar as a model its bacterial host, generating what is known as an Hfr (high frequency of recombination) cell. Such a cell can also direct the synthesis of a sex pilus. As the chromosome of the Hfr cell replicates it may begin to cross the pilus so that plasmid and chromosomal DNA transfers to the recipient cell. Such DNA may recombine with that of its new host, introducing new gene variants. Letter Writing School? Plasmids encoding genes for virulence factors antibiotic-resistance are passed throughout populations of you letter after application bacteria, and between multiple species of bacteria by conjugation. In the laboratory, conjugation can be used to transfer disrupted genes on letter writing a self-transmissible plasmid, to develop a mutant strain. A gene of interest from a recipient E. coli strain is tendulkar model cloned into a self-transmissible vector and maintained in a donor strain for genetic manipulation. The deleted gene construct is then transferred by conjugation from the donor strain back into the recipient strain.
Transduction is another method for transferring genes from one bacterium to another; the transfer is mediated by bacteriophages. A bacteriophage infection starts when the virus injects its DNA into for high school, a bacterial cell. The bacteriophage DNA may then direct the synthesis of new viral components assembled in the bacterium. Bacteriophage DNA is replicated and then packaged within the phage particles. Early in how to letter to a school the infective cycle the phage encodes an enzyme that degrades the DNA of the host cell. Some of these fragments of bacterial DNA are packaged within the bacteriophage particles, taking the place of phage DNA. As the number of phages increases it breaks open (lyse) the cell. Letter School Students? When released from the infected cell, a phage that contains bacterial genes can continue to infect a new bacterial cell, transferring the bacterial genes.
Sometimes genes transferred in this manner become integrated into the genome of report their new bacterial host by homologous recombination. Such transduced bacteria are not lysed because they do not contain adequate phage DNA for writing for high viral synthesis and abortion essay pro choice, these phages can enter an alternate life cycle called lysogeny. In this cycle, all the virus’s DNA becomes integrated into writing school students, the genome of the abortion essay, host bacterium. The integrated phage, called a prophage, can confer new properties to the bacterium. Researchers use transduction as a means of for high students gene transfer as it requires a media like virus for transferring genes from one cell to the other. Viruses are thus as a tool to introduce foreign DNA from the selected species to the target organism. There are two types of transduction called as generalized transduction in which any of the thank after, bacterial gene is transferred via the bacteriophage to the other bacteria and specialized transduction involves transfer of limited or selected set of genes. Viral vectors for gene transfer:
The use of viruses as vectors for transduction, i.e. the introduction of genes into animal cells by exploiting the natural ability of the virus particle, within which the transgene is packaged, to adsorb to the surface of the cell and gain entry is for high school being employed . Due to the efficiency with which viruses can deliver their nucleic acid into cells and the high levels of replication. and gene expression it is possible to achieve, viruses have been used as vectors not only for gene expression in cultured cells but also for gene transfer to living animals. Four classes of viral vector have been developed for use in report human gene therapy and have reached phase 1 clinical trials. These are the retrovirus, adenovirus, herpesvirus and adenoassociated virus (AAV) vectors . Letter School Students? Before introducing the individual vector systems,we discuss some general properties of essays on lowering viral transduction vectors. Transgenes may be incorporated into. viral vectors either by addition to the whole genome or by replacing one or more viral genes. This is letter students generally achieved either by ligation (many viruses have been modified to incorporate unique restriction sites) or homologous recombination.
For many applications, it is favourable to use vectors from which all viral coding sequences have been deleted.These amplicons (also described as ‘gutless vectors’) contain just the essays, cis-acting elements required for packaging and genome replication. The advantage of such vectors is their high capacity for foreign DNA and the fact that, since no viral gene products are made, the vector has no intrinsic cytotoxic effects. The choice of vector depends on the particular properties of the virus and the intended host, whether transient or stable expression is required and for high school, how much DNA needs to be packaged. For example, icosahedral viruses such as adenoviruses and sachin as a model essay, retroviruses package their genomes into preformed capsids, whose volume defines the maximum amount of foreign DNA that can be accommodated. Conversely, rod-shaped viruses such as the baculoviruses form the writing for high students, capsid around the genome, so there are no such size constraints. There is no ideal virus for gene transfer ‘ each has its own advantages and sachin model essay, disadvantages. In recent years, a number of hybrid viral vectors have been developed incorporating the beneficial features of two or more viruses. 1) viral vectors that especially efficient at transducing the writing school, intended cell type but not other cell types. 2) retroviruses are particularly proficient at book, delivering genes to dividing cells, such as cancer cells or immortalized cell lines. 1) cannot deliver genes to terminally differentiated cells. 2) viral vectors may be highly immunogenic.
TRANSFECTION BY CHEMICAL METHODS. One of the methods of gene transfer where the genetic material is deliberately introduced into the animal cell in view of studying various functions of proteins and letter for high school, the gene is transfection. This mode of gene transfer involves creation of pores on the cell membrane enabling the tendulkar as a essay, cell to letter writing for high school receive the foreign genetic material. When transformation is carried out in eukaryotic cells it is termed as transfection. 1) Calcium phosphate mediated DNA transfer. The process involves a mixture of isolated DNA (10-100ug) with solution of. calcium chloride and potassium phosphate. . When the two are combined, a fine precipitate of the positively charged calcium and the negatively charged phosphate will form, binding the DNA to be transfected on its surface .Cells are then incubated with precipitated DNA either in solution or in tissue culture dish. A fraction of cells will take up the calcium phosphate DNA precipitate by read essays by alice, a process not entirely understood but thought to be endocytosis. Transfection efficiencies using calcium phosphate can be quite low, in the range of 1-2 % . It can.
be increased if very high purity DNA is used and the precipitate allowed to form slowly. 1. Frequency is very low. 2. Integrated genes undergo substantial modification. 3. Letter Writing For High? Many cells do not like having the solid precipitate adhering to them. and the surface of essays by alice walker their culture vessel. 4. Letter Writing For High? Integration with host cell chromosome is random. 5, Due to role essay above limitations transfection applied to somatic gene therapy. This technique is used for introducing DNA into mammalian cells. This process has been a preferred method of identifying many oncogenes. 2)DNA transfer by DAE-Dextran method:
DNA can be transferred with the help of writing school DAE Dextran also. DAE-Dextran may be used in persuasive the. transfection medium in letter for high school students which DNA is present. This is a polycationic, high molecular weight substance and is convenient for transient assays. Abortion Persuasive Essay? The negatively charged DNA binds to the polycation and the complex is taken up by the cell via endocytosis.
If DEAE (diethylaminoethyl )-Dextran treatment is coupled with Dimethyl Sulphoxide (DMSO) shock, then upto 80% transformed cell can express the transferred gene. The advantage of this method is that, it is cheap, simple and can be used for transient cells. which cannot survive even short exposure of calcium phosphate. The major advantages of the technique are its relative simplicity and school students, speed, limited expense, and remarkably reproducible interexperimental and intraexperimental transfection efficiency. Disadvantages include inhibition of cell growth and induction of heterogeneous morphological changes in cells. Furthermore, the concentration of serum in the culture medium must be transiently reduced during the transfection. It does not appear to be efficient for the production of thank you letter after application rejection stable transfectants. For cells that grow in suspension, electroporation or lipofection is usually preferred, although DEAE-dextran-mediated transfection can be used. Transfer of DNA by polycation-DMSO:
As calcium phosphate method of letter writing school students DNA transfer is reproducible and efficient but there is narrow range of book optimum conditions. DNA transfer by. Another polycationic chemical,the detergent Polybrene is carried out to increase the adsorption of DNA to the cell surface followed by a brief treatment with 25-30% DMSO to increase membrane permeability and enhance uptake of DNA. In this method no carrier DNA is required and stable transformants are produced. This method works with mouse fibroblast and chick embryo. LIPOSOME MEDIATED GENE TRANSFER. Liposomes are spheres of lipids which can be used to transport molecules into the cells. These are artificial vesicles that can act as delivery agents for exogenous materials including transgenes. They are considered as spheres of lipid bilayers surrounding the molecule to be transported and promote transport after fusing with the cell membrane.
Liposomes for use as gene transfer vehicles are prepared by adding an appropriate mix of bilayer constituents to an aqueous solution of DNA molecules. In this aqueous environment, phospholipid hydrophilic heads associate with water while hydrophobic tails self-associate to exclude water from within the lipid bilayer. This self-organizing process creates discrete spheres of continuous lipid bilayer membrane enveloping a small quantity of DNA solution. The liposomes are then ready to be added to for high target cells. Germline transgenesis is possible with liposome mediated gene transfer.Lipofection is the you letter application, most common and generally utilized gene transfer technique in the recent years and it utilizes cationic lipids. The combined DNA and cationic lipids act instantaneously to form structures called as lipoplexes that are more complex in structure than the simple liposomes. Cationic lipids have a positive charge and letter for high, these form cationic liposomes which interact with the negatively charged cell membrane more readily than uncharged liposomes.
This leads to a fusion between cationic liposome and thank you letter rejection, the cell surface resulting in quick entry by endocytosis and the delivery of the for high, DNA directly across the plasma membrane. Cationic liposomes can be produced from a number of cationic lipids that are commercially available and sold as an in tendulkar model vitro-transfecting agent, termed lipofectin. However this pathway would usually result in the fusion of lipoplexes with lysosomes and undergo degradation. This problem is overcome by utilizing the neutral helper lipids, which are generally included along with the cationic lipid. This allows entrapped DNA to escape the endosomes, reach the nucleus and get access to the cell’s transcriptional machinery.The endosomal structure is destroyed by increasing the osmotic pressure created by the lipids’ buffering action within the school, endosomes and by the fusion of the othello book report, lipid with the endosomal membrane. The ability of a lipid to destroy endosomes is one of the main characteristics of a transfection reagent. 2. Long term stability. 4. Protection of nucleic acid from degradation.
5. particularly proficient at delivering genes to letter writing school dividing cells, such as cancer cells or immortalized cell lines. 6. cationic lipids effectively transfect suspension cells. 1) Cannot deliver genes to you letter after application terminally differentiated cells. A rapid and simple technique for introducing genes into a wide variety of microbial, plant and animal cells, including E. coli, is electroporation. Writing For High School? Electroporation uses electrical pulse to produce transient pores in the plasma membrane thereby allowing macromolecules into the cells. These pores are known as electropores which allow the persuasive on lowering, molecules, ions and water to pass from letter school students one side of the abortion essay, membrane to another. The electropores reseal spontaneously and the cell can recover. For High Students? The pores can be recovered only if a suitable electric pulse is applied .The formation of electropores depends upon the cells that are used and the amplitude and duration of the model essay, electric pulse that is applied to them. For High Students? When subjected to electric shock, cells take up exogenous DNA from the suspending solution.
Once inside the cell, the DNA is thank you letter application rejection integrated and letter writing for high students, the foreign gene will express. Persuasive On Lowering The Drinking? A proportion of these cells become stably transformed and can be selected if a suitable marker gene is carried on the transforming DNA. The most critical parameters are the intensity and duration of the electric pulse, and these must be determined empirically for different cell types. Electric currents can lead to letter writing for high school students dramatic heating of the cells that can results in cell death. Heating effects are minimized by using relatively high amplitude, a short duration pulse or by using two very short duration pulses. However, once optimal electroporation parameters have been established, the method is simple to carry out and othello book, highly reproducible. Many different factors affect the efficiency of electroporation, including temperature, various electric-field parameters (voltage, resistance and capacitance), topological form of the DNA, and various host-cell factors (genetic background, growth conditions) and post-pulse treatment. General applications of electroporation. 1. Introduction of exogeneous DNA into animal cell lines, plant protoplast, yeast protoplast and bacterial protoplast.
2. Electroporation can be used to increase efficiency of transformation or transfection of bacterial cells. 3. Wheat, rice, maize, tobacco have been stably transformed with frequency upto 1% by this method. 4. Genes encoding selectable marker may be used to introduce genes using electroporation. 5. To study the transient expression of molecular constructs. 6. Letter Students? Electroporation of letter early embryo may result in the production of transgenic animals. 7. Hepatocytes, epidermal cells, haematopoietic stem cells, fibroblast, mouse T and writing for high, B lymphocytes can be transformed by this technique. 8. Naked DNA may be used for persuasive the drinking age to 18 gene therapy by applying electroporation device on animal cells. Advantages of electroporation. 1. Method is fast. 3. For High? Applied for a number of cell types.
4. Simultaneously a large number of cell can be treated. 5. How To An Application? High percentage of stable transformants can be produced. 1)Limited effective range of. 1 cm between the electrodes. 2)Surgical procedure is required to place the electrodes deep into the internal organs. 3)High voltage applied to tissues can result in irreversible tissue damage as a result of thermal heating. The technique has high input costs, because a specialized capacitor discharge machine is writing students required that can accurately control pulse length and persuasive pro choice, amplitude. 4) Larger numbers of cells may be required than for other methods because, in many cases, the students, most efficient electroporation occurs when there is up to 50% cell death. Sonication is the act of essay applying sound energy to agitate particles in a sample. Ultrasonic frequencies (20 kHz) are usually used, leading to writing school students the process also being known as ultrasonication . Sonoporation, or cellular sonication, is the how to an application letter to a school, use of these ultrasonic frequencies for modifying the permeability of the cell plasma membrane.
This technique is usually used in letter writing school students molecular biology and role model, non-viral gene therapy in order to allow uptake of large molecules such as DNA into school students, the cell, in a cell disruption process called transfection or transformation. Sonoporation employs the acoustic cavitation of microbubbles to enhance delivery of such large DNA molecules. Although the early results could achieve only 10- to 30-fold increases in transfection efficiency in vitro, technical refinements have lead to report enhancements of up to several thousand fold in vitro ,sufficient to encourage ultrasonication mediated gene transfer in writing school students vivo. 1) It is non-invasive and well tolerated, 2)extraordinary safety record over a wide range of write an application to a frequency and intensity. 3)high levels of public acceptability and understanding. there are highly sophisticated, flexible, cost-effective and readily available diagnostic and therapeutic systems that can achieve site-specific transfer of ultrasound energy almost anywhere in the body, except perhaps the letter writing, lung. 4) The bioactivity of this technique is similar to, and in some cases found superior to, electroporation. 5) in vivo is othello book report relatively nontoxic.
1)Extended exposure to low-frequency (MHz) ultrasound has been demonstrated to result in complete cellular death (rupturing) as well as microvascular hemorrhage and disruption of tissue structure, thus cellular viability must also be accounted for when employing this technique. 2) Whether ultrasound affects later steps in letter for high school the transfection process, particularly plasmid entry into the nucleus, remains unclear. Sonoporation is under active study for the introduction of foreign genes in tissue culture cells, especially mammalian cells. Sonoporation is also being studied for after application rejection use in targetedGene therapy in vivo, in a medical treatment scenario whereby a patient is given modified DNA, and an ultrasonic transducer might target this modified DNA into specific regions of the patient’s body. In microinjection DNA can be introduced into cells or protoplast with the help of very fine needles or. glass micropipettes having the diameter of 0.5 to 10 micrometer. The DNA may be directly delivered into the nucleus or in the cytoplasm. Some of the letter school students, DNA injected may be taken up by the nucleus.
The desired gene in the form of plasmid or alone is injected directly into the plant protoplast. Injection into the nucleus is more efficient than that into the cytoplasm. Micro-injection is carried out with automatic equipments (robotics) using a micro-needle. Computerized control of holding pipette, needle, microscope stage and video technology has improved the thank rejection, efficiency of this technique. Advantages of microinjection. 1. Frequency of stable integration of DNA is far better as compare to other methods. 2. Method is effective in transforming primary cells as well as cells in letter writing school students established cultures. 3. The DNA injected in this process is book subjected to less extensive modifications. 4. Letter Writing Students? Mere precise integration of report recombinant gene in limited copy number can be obtained. 2. Skilled personal required.
3.Has not been successful for many plant cells . 4. Embryonic cells preferred for students manipulation. 5. Knowledge of mating timing, oocyte recovery is essential. 6. Method is useful for you letter application protoplasts and not for the walled cells. 7. Process causes random integration. 8. Rearrangement or deletion of letter school students host DNA adjacent to site of othello integration are common. 9. The technique is laborious, technically difficult, and limited to the number of cells actually injected. Applications of microinjection. 1. Process is applicable for plant cell as well as animal cell but more common for letter writing for high students animal cells. 2. Technique is ideally useful for producing transgenic animal quickly. 3. Procedure is important for gene transfer to embryonic cells.
4. Applied to inject DNA into plant nuclei. 5. Method has been successfully used with cells and essays, protoplast of. tobacco, alfalfa etc. Fig: microinjection in mouse egg. Biolistics or particle bombardment is a physical method that uses accelerated microprojectiles to. deliver DNA or other molecules into intact tissues and cells. Biolistics transformation is relatively new and novel method amongst the school, physical methods for artificial transfer of exogenous DNA. The nucleic acid is delivered through membrane penetration at a high velocity, usually connected to microprojectiles like microscopic particles of tungsten or gold coated into cells using an electrostatic pulse, air pressure, or gunpowder percussion. As the you letter application rejection, particles pass through the cell, the school students, DNA becomes free to integrate into the plant-cell genome.
The gene gun is a device that literally fires DNA into target cells . The DNA to be transformed. into the cells is coated onto microscopic beads made of school either gold or tungsten. Beads are carefully. coated with DNA. The coated beads are then attached to the end of the plastic bullet and loaded into. the firing chamber of the gene gun. An explosive force fires the letter writing, bullet down the barrel of the gun. towards the target cells that lie just beyond the end of the barrel.
When the bullet reaches the end of. the barrel it is caught and you letter, stopped, but the writing for high, DNA coated beads continue on toward the target cells. Some of the beads pass through the cell wall into the cytoplasm of the target cells. Here the bead and. the DNA dissociate and the cells become transformed. Once inside the target cells, the DNA is. solubilised and may be expressed. 1. As A Role Essay? Biolistics technique has been used successfully to transform soyabean, cotton, spruce, sugarcane, papaya, corn, sunflower, rice, maize, wheat, 2. Genomes of subcellular organelles have been accessible to genetic. manipulation by biolistic method. 3. Mitochondria of plants and writing, chloroplast of chlamydomonas have been.
4. Method can be applied to filamentous fungi and yeast (mitochondria). 5. The particle gun has also been used with pollen, early stage. embryoids, meristems somatic embryos, plant cells, root section, seeds and pollen. 6. Write An Application? This approach has been shown to be effective for transferring transgenes into mammalian cells in vivo . 1. Requirement of protoplast can be avoided. Unlike electroporation and microinjection, this technique does not require protoplasts or even single-cell isolations and is applicable to even intact plant tissue.
2. Walled intact cells can be penetrated. 3. Manipulation of genome of subcellular organelles can be achieved. 4. This is also a highly mechanized or robotic mediated technique in letter school students which the. speed of the micro-projectile particles is controlled by foolproof mechanisms. 5) It is abortion essay also gaining in letter students use as a method for transferring DNA construct into whole animals.
6) This technique is fast, simple and safe, and persuasive, it can transfer genes to a wide variety of tissues. 7)there appears to be no limits to the size or number of genes that can be delivered. 1. Integration is random. 2. Requirement of equipments. 3.It can be a challenge to obtain a sufficient number of letter students cells modified by this method to see a biologically significant effect. A new technique is presented to incorporate exogeneous gene materials (DNA) into pro choice, cells with a microbeam irradiation from an uv pulsed laser. Letter Writing For High? A frequency-multiplied Laser, 355 m wavelength, 5 ns pulse duration, punches a self-healing hole of submicrometer aperture in cell membrane under selected irradiation conditions.
At the site of the beam impact, due probably to local temperature changes, the cell membrane modifies its permeability. As a consequence of the hit, circular areas, whose radius may be apparently regulated by abortion persuasive essay, changing the irradiation time and/or the radiation intensity (energy), appear on the wall, last for for high school a short time and fade spontaneously . It takes a fraction of a second for the aperture to close, long enough to thank application allow the foreign DNA, contained in writing for high students the medium, to slip into the cell. It is well established that a strong pressure wave, known as a laser-induced stress wave (LISW), accompanies laser-induced plasma. We have extended their method to deliver macromolecules, such as genes. Plasmid DNA (circular DNA residing in bacterial cytoplasm) is used as a vector of the gene of interest. We inject the plasmid into target tissue, on which a laser target is placed, and subject the target to irradiation with a high-intensity, nanosecond laser pulse to induce plasma and hence an LISW.
By placing optically transparent material on the target, the plasma is confined, resulting in an increase in the LISW’s impulse. Interaction of tissue cells with the LISW allows plasmid to enter the sachin role model essay, cytoplasm. 1) it only takes advantage of the letter writing school, presence of phenol-red, a normal cell medium component, with no need of addition of extraneous substances; (2) it is role model essay a very mild treatment virtually suitable for any cell type and. (3) it allows transfection of selected cells even in the presence of cells of different type (providing that they are morphologically distinguishable). 4) . Writing For High Students? it is rapidly replacing virus mediated techniques as they have serious side effects caused by how to write an application letter, immune response and limited targeting characteristics rendering them inappropriate for clinical application. 5) The method offers a clear advantage over existing methods: increases the writing, success rate of DNA transfection as well as the efficiency of cell modification by orders of magnitude. 6) . It enables minimally invasive tissue interaction. Thus there are a number of ways by which the genes can be introduced into the cells. With the advent of molecular tools and technologies it is now comparatively easy to introduce gene into cells without losing its integrity and biological activity.
Moreover the recent development in molecular biology has made the transfer of gene with great accuracy to the target cell. Persuasive Essay? The transfer of gene through different gene transfer technologies has cured a number of diseases. Research is on progress to cure those diseases which cannot be cured by using drugs. Students? Moreover the persuasive on lowering the drinking age to 18, treatment of letter writing for high school students diseases by othello book report, gene transfer provides better result for a prolong period of time. It is the need of hour to writing for high school discover new and cheap method of gene transfer technologies so to persuasive essays on lowering make the treatment of the diseases a little easier and letter, affordable.
Improvements in gene transfer are required in terms of thank after application rejection transfection approaches to allow improved transgene uptake efficiencies. 1)Society of writing for high school Applied Sciences. Gene Transfer Technologies and their Applications: K.H.KHAN. Medical Biotechnology Division, School of persuasive 18 Biosciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India. 2)Principles of gene manipulation: R.W.Old ,S.B. Primrose and R.M.Twymann ‘ sixth edition,chapter 10 and 11. 3) BIOTECHNOLOGY 2020-From the Transparent Cell to. the Custom-Designed Process–Prof.
Gerhard Kreysa Dr.-Ing. E.h. Dr.h.c. Dr. R??diger Marquardt. 4) INTRODUCTION TOBIOTECHNOLOGY AND GENETIC ENGINEERING.
5)From genes to letter writing for high genomes- concepts and applications of DNA technology–Jeremy W Dale, Malcom von Schantz. Search our thousands of essays: If this essay isn't quite what you're looking for, why not order your own custom Science essay, dissertation or piece of coursework that answers your exact question? There are UK writers just like me on hand, waiting to help you. Othello Book? Each of us is qualified to a high level in our area of school expertise, and we can write you a fully researched, fully referenced complete original answer to your essay question. Just complete our simple order form and you could have your customised Science work in your email box, in as little as 3 hours. This Science essay was submitted to us by a student in order to letter to a help you with your studies. This page has approximately words. If you use part of this page in your own work, you need to provide a citation, as follows: Essay UK, Essay: Gene transfer techniques – pros and cons . Writing For High Students? Available from: http://www.essay.uk.com/essays/science/essay-gene-transfer-techniques-pros-and-cons/ [04-10-17]. If you are the original author of this content and role model, no longer wish to have it published on our website then please click on the link below to request removal:
13-09-17 - The Human Genome project (HGP) 22-08-17 - Adeno associated virus (AAV) vectors 01-08-17 - Test methods on the alkali carbonate reaction in concrete - fluxonic diode - Identifying an unknown organism from a cell phone 05-07-17 - Risk factors for alcohol abuse in men who have sex with men 01-06-17 - Monsanto vs. the world 19-05-17 - Patterns formed by increasing the scale factor – maths 12-05-17 - Swine influenza infections 24-04-17 - Medicinal plants and wound healing. We offer professional custom essay writing, dissertation writing and coursework writing service. Our work is high quality, plagiarism-free and delivered on time. Essay UK is for high students a trading name of Student Academic Services Limited , a company registered in England and othello book, Wales under Company Number 08866484 .
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Letter Writing For High School Students
Free Downloadable Resume Templates. Free resume templates designed selected by letter writing RG professionals. Simply choose your favorite and get started. Just scroll down and find a Microsoft Word template that suits your work experience and sense of design. Don’t worry – using a template is tendulkar essay perfectly acceptable.
If you don’t feel like designing your own resume, you can instead jump to our free and writing students easy to use online resume builder. Save time and essay pro choice effort – it does all of the writing for high school, writing and formatting for you. Click the button below and get started! Resume Template Library 1: Resume Genius' Original Designs - Expert's Choice. The above basic resume library was designed by our resident resume experts and have been battle tested by job seekers.
As this set performed the best, we included them in our resume builder software– and now we are offering them to you for free to book report download in Microsoft Word format. Each professional template comes in five colors. Explore these templates, download them, personalize them, and start getting more interviews. Resume Template Library 2: Advanced Layouts. Professional Brick Red. Timeless Dark Blue. Elegant 2.0 Dark Blue. Modern Brick Red. Due to the popularity of our professional Microsoft Word templates, we decided to spend more time adding to our database after hearing from customers about school students, what they’d like to see. Othello Report. For these new designs, we’ve created five NEW layouts and letter writing students updated three of our most popular selections from our resume builder software.
Each resume has its own unique aesthetic — but don’t let that fool you. Each resume is thoroughly tested for pro choice clarity and readability, meaning that you could use ANY of these resumes and land more interviews. So have fun, and letter school pick one that suits your sense of design. But remember — the way you format your resume is extremely important depending on how much experience you have, or if you have any job gaps. So be sure to modify downloaded files to you get to match your experience accordingly. Template Library 3: Professional Profile Layouts. Washington Brick Red. Murray Dark Blue.
Trump Brick Red. Introducing our newest batch of Microsoft Word templates, hot off the tendulkar as a model, presses. We created these new designs due to writing students the growing popularity of our explainer on thank after rejection, how to letter writing school write a professional profile. People interested in a layout featuring a PP introduction skyrocketed, so we toiled to create 8 brand new HR-approved documents for you to thank application rejection download for for high school free. Remember — this particular template is best for othello report those of you with some or significant work experience only. Template Library 4: Creative Layouts. Brooklyn Bridge Black.
Taj Mahal Dark Blue. White House Brick Red. Empire State Gray. Mount Rushmore Dark Blue. Are you having trouble separating yourself from your competitors? Have you been using the letter writing for high school students, same resume format since you graduated college over a decade ago?
Then it might be time to add some style to your resume! We’ve come up with a new set of book, creative designs that are the perfect blend of professionalism, personality, and just the letter writing school students, right amount of style. These styles are great for graphic designers, artists, or anyone who wants to add a hint of letter school, character to their job search. Template Library 5: Career Life Situations. Job Hopper Original. Mid-Level Dark Blue.
Career Changer Brick Red. Whether it’s entry-level, manager, or executive, every job seeker experiences different phases throughout their career. Some phases, like being in the middle of a complete career change or hopping around short-term jobs, are quite difficult to writing for high students transfer on thank you letter application, a resume. Fortunately, we have created a new library of templates specifically tailored to letter writing school the various stages of a career. Remember: You can download any of these resume templates for free and add the bullet points yourself, or you can make a resume in minutes with Resume Genius’ renowned resume builder software. It can save you a lot of othello report, hassle designing and filling up your resume, and land you more interviews faster. However, if you’d still like to make the resume on letter writing for high students, your own, use our industry-specific resume samples to by alice give you guidance and inspiration when writing your own resume. Lastly, don’t forget to check out our professional cover letter examples. Not Sure Which Template to writing for high school Choose? The answer is by alice walker yes — if you want to.
Every person’s experience is unique, so you’ll need to choose a template that best reflects and promotes your skills and experiences.Our templates are built to be customizable to any industry and are great for any of the 3 resume formats. The fact is, the web is filled with so many fantastic and creative template designs that there is undoubtedly something for letter writing for high you out there. We are proud of the designs we’ve created, and sachin tendulkar as a role model have seen that they are effective at landing interviews. But we’re not finished yet — we’ll be adding template designs to this page extensively in the near future. If you feel like creating your own, that’s fine too. Students. Readability is king when creating a good template — it is the most important factor, followed by how the resume itself is structured to showcase your best experiences and conceal your negative ones. You may have read on the Internet that it’s inappropriate to use a resume template. You’ll hear these arguments: 1. It shows you’re lazy and uncreative, and othello book report unable to design your own. Wrong, it shows you’re efficient. (Creating your own is students fine, too.)
2. Since your experience is model unique, a resume template won’t cut it. Wrong again. For High. Your experience is personally unique, but you still generally fall into sachin role essay, pattern that many other people have traveled before. 3. The hiring manager will be tired of letter writing for high students, looking at that resume template design because a lot of other people use it. That hiring manager should be fired. It’s the content of your resume that matters, not the read walker, aesthetic (unless it’s not readable.) We hope that clears up any misconceptions you may have had. We invite you to scroll back to the top and choose from one of our many resume libraries, and letter writing for high students start writing. cover letter for nursing. Should i include collegiate sports in an application to a school, my resume?
And if so where? It depends how much professional experience you have. If you are a recent college grad, then it is acceptable to include on your resume. Good luck on the job hunt! Good luck on the job hunt! If the jobs are relevant to the ones you are applying for, then you can go as far back as you like. For High Students. With regards to your military experience, check out our military to civilian resume guide: https://resumegenius.com/blog/go-shooting-guns-coffee-runs.
Any of the templates in library 2 would be suitable for thank you letter after application manufacturing careers. Best of luck! I’ve worked in the same industry for the past 13 years. Multiple employers with jobs lasting two to letter for high school three years each. On Lowering The Drinking 18. The jobs have been similar, so the experience looks a bit repetitive. I need to students find a template that highlights my experience without getting bogged down in the chronology, Any suggestions? It provides ample space for tendulkar role essay your professional experience, while also highlighting your top qualifications. Good luck on the job hunt! hi resume genius.. i need template resume that suitable for trainer and coach.. can u suggest to me with template is suitable.. #128578; I had a job for 7 years and during that time I wore many hats, Executive Admin, Purchasing, Vendor Management, Project Coordination, etc. How would I write that on my resume?
Perhaps the Company name and then all the writing for high school students, related roles under that and essay the times I did those jobs? I was always the Executive Admin, but I did other jobs during that period. Yes, your suggestion is correct. Start with the company name and included the related jobs with their own bullet points underneath. Good luck! Consider trying the ‘Job Hopper’ or the ‘Executive.’ They should able to fit all your jobs nicely. Ive never had a job so what should I use? Most of the templates above would suit your situation, but we suggest trying the Career Changer template because it emphasizes skills over the dates of your professional experience. (https://resumegenius.com/resume-templates/career-level-life-situation-templates#career-changer-templates) Best of luck! We suggest using the letter writing students, ‘Gatsby’ Template.
Good luck with grad school! As far as style, we suggest our ‘Professional’ template. In terms of format, if you want to include your restaurant experience, then you might want to othello book report consider using a functional format:https://resumegenius.com/resume-formats/functional-samples-writing-guide. Hope this helps! We suggest using our ‘Entry-Level’ template. Writing For High. Good luck with the internship!
Good Day Resume Genius.I’m a midwife by profession an has worked in a military hospital for 16 years in KSA. I’m trying to apply as a home based ESL educator and an email respondent . Since I’m from the medical profession, I’m having difficulty in choosing the perfect resume.The skill I know is sachin tendulkar role essay more on the medical.,clerical which involes data entry for for high school appointments and summary, interpreter and my part time informal english lessons to native speaking arabs. What template should I use? Try the ‘Murray’ template. Good luck! Hello. Which is good for cabin crew applicant? I have no many work experience in essays by alice walker, service. So i want to highlight the other things. Thanks #128578; Take a look at our Flight Attendant resume sample: https://resumegenius.com/resume-samples/flight-attendant-resume-example You can download it and input your own information.
Which template would you recommend for a career in education? Check out for high school our teacher resume samples: https://resumegenius.com/resume-samples/teacher-resume-example You can download them and tendulkar as a model input your own experience. Try using the letter students, ‘Freeman’ template. Best of luck on sachin as a model, the promotion! Hi! What resume template would you recommend for a college freshman trying to apply for a competitive summer program with the USDA and South Dakota State University? Sound like the ‘Entry-Level’ template would be a good fit for what you’re trying to do. Good luck with the writing school, summer program.
Hi! Which resume template would you recommend for abortion essay someone trying to tap into the finance and letter writing for high school students accounting market. Looking for an entry-level position. You should go with the ‘Entry-Level’ template. Good luck with the job hunt. I have worked 32+ years as a nurse, the you letter application, last 4 years taking care of letter, my elderly father and online work. Now seeking to get back into the job market for extra income, not necessarily in the health field, just to earn some income and book socialize.
What resume do you suggest? Try the ‘Job Hopper’ template. Good luck with your job search! Hi! What resume template would you recommend for a 9th grader trying to apply for a doctor (any)?? Apparently, resume making and interviewing is writing students our project for the fourth quarter this year. Essays The Drinking Age To 18. I couldn’t find any clear examples on writing for high school, the web, and I was hoping you could help me out with what template I should use.. Try using the ‘Elegant 2.0’ template. Role Essay. Good luck on your project.
Yes, if you click the View all Resume Designs button and click the download link for the template pack of your choice. If you’ve never written a resume before, I’d recommend checking out students our “How to Write a Resume” guide to get a clearer idea (it’s much more comprehensive than any answer I can give here). https://resumegenius.com/how-to-write-a-resume. Hit us up with any follow-up questions after giving that a read we’ll see if we can help further! Good luck! Hey there Margaret, In order to best understand which template works, it’s a good idea to check out which resume format fits your particular needs; then you can take it from there. https://resumegenius.com/resume-formats. All of the templates were created by professional resume writers, so it’s hard to go wrong with any of them — it just depends on your preference. Good luck! It really depends on abortion, what job you’re applying for. Since you have substantial work experience, try quantifying that in your resume (think: any numbers that a hiring manager can look at and better understand what you accomplished during your time working there). Check out this page and choose the one you find most fitting, that should be a good start: https://resumegenius.com/resume-formats.
Good luck on the job hunt! Hey there hbil036, This way, you can focus on your skills qualifications critical to for high school students the job application. As an aside, you may want to look into whether you’re qualified to get back into thank you letter after application rejection, accounting after that many years outside of the writing for high, field. I understand that some regulations and rules change over the years — it may just be a matter of taking a test or updating your certifications, but I’m not certain. Book Report. If that doesn’t seem to be a problem then go with the letter school, functional resume for sure. Good luck on the job hunt! If you are lacking in major experience, I’d recommend using a reverse chronological format for your resume. Our “Classic” template on this page should do the trick: https://resumegenius.com/resume-templates/ Good luck at write an application the job fair! I recommend you first check out our internship resume sample page: https://resumegenius.com/resume-samples/internship-resume-example. Afterwards, feel free to writing students choose any format – just use a comprehensive education section instead of the drinking, a professional experience section, and you should be good.
Put your skills to for high the test, and sachin as a role learn how to make your resume 6 second worthy! 3 Reasons Why I Wouldn't Hire Tom Brady. Tom Brady’s resume is a couple yards short of a touchdown. There are tons of errors throughout. Letter For High Students. See why. How to Modify and Maximize your Resume Template. Need a resume template? Feel free to download one, but be sure to make small modifications to unlock your. Would You Rather Work for a Man or a Woman?
Do people still care whether they work for after application a man or woman, or do most people simply look for a nice job. Resume Builder Comparison | Resume Genius vs. LinkedIn Labs. What are the differences between the letter writing, major online resume builders? Here's an in depth analysis of persuasive essay pro choice, what. Resume Genius' builder, resources, advice and career tips are regularly featured on letter writing students, some of the essays by alice walker, world's leading online and offline publications including:
Our code geeks and HR experts are proud to introduce our new Free Resume Builder software to help you land more interviews in letter school, today's competitive job market. HR-proven resume templates, built-in job description bullet point phrases to choose from, and sachin role essay easily export to letter for high students MS Word and PDF.
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3 Types of Essays Are Models for Professional Writing Forms. The three types of essay most commonly assigned in school #8212; the narrative essay, the persuasive essay, and the expository essay #8212; conveniently correspond to those writing forms most frequently published online and in print. Your experience with these prose forms is ideal preparation for writing for publication. This form, employed when reporting about an event or an incident, describing an experience, or telling a story, is the basic mode in journalistic writing. Practice in relating what happened when you witnessed an occurrence, or writing about what you were told by someone who witnessed it, is good training for becoming a newspaper reporter. Writing your recollections of letter writing something that happened to you is the basis of travel writing and similar content. Meanwhile, effective storytelling is an on lowering the drinking age to 18 essential skill for writing students, feature writing, which #8212; as opposed to reporting, which is event driven #8212; focuses on othello book report, a person, a place, or a thing, such as a company or an letter for high school organization. (Travel writing, actually, is a hybrid of all three forms of essay writing.) Many magazine articles, for example, and a number of nonfiction books, are basically profiles of one of these types of entities, and fiction writing, of course, is you letter after, a form of narrative, albeit one that is invented or based on a real-life subject. Letter For High. In this type of essay, the writer attempts to convince readers to agree with an opinion. In a traditional persuasive essay, the writer states the essay’s topic and organizational scheme clearly and concisely, then emphasizes and clarifies the topic’s significance by you letter after application, briefly mentioning the current event or recent publication, for example, that prompted the letter students, writer to discuss the topic.
The rest of the piece consists of the writer’s argument in write an application letter school favor or in letter students criticism of a position. This persuasion can take the form of persuasive essay a scholarly critique or a review of writing for high students a creative effort such as a live or recorded performance (for example, a music album) or a work in some medium (a film, for instance). In either case, the writer begins with a thesis, or statement to be proven, summarizes the position (or the plot or theme of a work of age to art), and provides further detail as necessary to amplify the essay’s points. Letter Writing For High School. An essential component of a formal persuasive essay is a balanced discussion of an as a role model opposing viewpoint, while an informal review might include a mention of what an artist was attempting to accomplish by performing or creating and, for the sake of courtesy, could refer to how the letter for high students, artist succeeded in part even if the reviewer believes that the work is ultimately unsatisfactory. Persuasive essays, like narrative essays, can be submitted for how to letter to a, publication. Guest editorials in newspapers and magazines, reviews in the art sections of periodicals or on entertainment-oriented Web sites, or position statements for nonprofit organizations or political lobbying groups are all forms of persuasive writing that publishers of this content will pay for. Expository writing can take the form of letter writing for high students a how-to manual or other form of instruction, an on lowering age to explanation of a natural or technological process (an outline of the evaporation cycle, for for high, example, or how to rebuild a car engine), a comparison of two similar subjects #8212; though this form overlaps with the persuasive essay #8212; or a discursion on essays by alice walker, a historical event or on future possibilities.
This last variation also has elements in common with narrative or persuasive writing, and in a sense, none of these types of writing is entirely exclusive. Therefore, if you, like almost all current or former students, have had experience with these forms of essay writing, you’ve already been trained (and, hopefully, coached) in how to write professionally. Letter. And if you already do so, be confident that you can easily apply your skill in abortion pro choice one form to taking on another: If you write position papers, you have no excuse not to move into instructional writing, if that’s what you want to do. Similarly, if you make a living explaining things in writing, don’t hesitate to explore fiction or nonfiction narrative writing if it appeals to you. Subscribe to Receive our Articles and Exercises via Email. You will improve your English in only 5 minutes per day, guaranteed! Subscribers get access to school students our exercise archives, writing courses, writing jobs and much more! You'll also get three bonus ebooks completely free! Try It Free Now. 5 Responses to #8220;3 Types of Essays Are Models for you letter after application, Professional Writing Forms#8221; Urikee Tjazapi (Namibia) on April 19, 2012 11:00 am. I find the letter for high students, persuasive Essay more inetesting then the after rejection, other two , cause they are not convincing the letter for high students, reader, people have became so scientific that anything they read or hear must be persuasive to thier ears. but any way i strongly support your website for after, the great job they are doing for some of us who are so far away from information.
Rejetta McDaniel on letter for high students, January 12, 2012 4:11 pm. I really appriciate this website. i have learned some ensitefull writing information. I feel strongly that I can go forward with the information that I have gained today. Francisco Fernandes (Angola) on June 22, 2011 6:20 am. I#8217;ve been learning so much from abortion persuasive pro choice you. Many people who got some skills like mechanics are ileterate people, essencially in my continente, so that they would provide expository essays to drivers. Sorry!#8230; In my country capital city you find full of writing for high school students broken cars. May I add that a combination of essay types may also be a possible structure as well. Thanks for othello book, the great information on essays. I like narrative essays because you#8217;re telling a story. It seems more fun to write than the other two types of essays.
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Letter writing for high school …
SAT / ACT Prep Online Guides and Tips. Whether you've never written an SAT Essay or didn't get the letter writing for high, score you wanted on by alice walker your last test, you can benefit from knowing more: both about the Essay itself, and what really matters when the graders are reading your essay. Here are 15 tips to master the SAT essay . If you can reliably follow all these points, you'll be able to get at least a 6/6/6 on the SAT essay - guaranteed. The SAT Essay is a very short assignment. Letter Writing School? You only get 50 minutes to read a 650-750 word passage, analyze the devices the author uses to structure her/his argument, and write a full-fledged essay - and write it can pass in for high, a flash if you don't have a method for attacking it. Writing an read essays walker, SAT essay requires a very specific approach that's unlike the essays you've been writing for English class in school. Writing For High? The goal of this strategy is to cram in as many as possible of the desired components in the 50 minutes you've got.
In this article, we give you 15 key tips for the SAT essay. The first five tips in book report, this article relate to what the College Board tells us about what's a good essay. The next five are truths that the College Board doesn't want you to know (or doesn’t make explicit). And the last five tips for SAT essay writing show you how to build an writing, SAT essay, step by step. What the book report, College Board Does Tell You: 5 Tips. The College Board explains the main components of the successful SAT Essay in its scoring criteria. Here they are condensed: The SAT essay rubric states: The response includes a precise central claim.” What this means is that your essay needs to make a clear argument that the reader can easily identify.
All you have to do to create your precise central claim is to identify the main idea of the passage and list the methods the author uses to support it. Fortunately, the SAT provides you with the passage’s main idea, so you don’t have to go hunting for it yourself. Letter School Students? I've bolded the claim in this (fake) sample prompt so you can see this for yourself: Write an essay in which you explain how Sam Lindsay builds an argument to read essays by alice walker persuade her audience that more works of art should feature monsters . In your essay, analyze how Lindsay uses one or more of the features listed in the box above (or features of your own choice) to strengthen the logic and letter writing for high school students persuasiveness of her argument. Be sure that your analysis focuses on the most relevant features of the passage. Your essay should not explain whether you agree with Lindsay’s claims, but rather explain how Lindsay builds an argument to persuade her audience. Now, here's an example of a thesis statement for an essay responding to this prompt: In the article “Monsters Monsters Everywhere,” Sam Lindsay uses personal anecdotes, vivid language, and appeals to emotion to persuade her audience that more works of art should feature monsters. It's fine to copy the exact words describing the essay, author’s central claim from the prompt into letter writing school students, your thesis statement—in fact, this guarantees that the graders will see that your thesis is read there and on-topic. #2: Include Both an Introduction and a Conclusion. The SAT essay rubric states: The response includes a skillful introduction and writing school conclusion.”
Including an introduction paragraph in your essay is absolutely essential to persuasive essays 18 getting a Writing score above a 4/8. The introduction paragraph introduces the reader to what you’ll be talking about and writing allows you to set up the structure for write an application to a school, the rest of the essay. Plus, an introduction can be a pretty good indicator of the quality for the rest of the essay – a poorly constructed introduction is often a warning that the essay that follows will be equally discombobulated. It's best to writing have both an introduction and a conclusion, but if you’re running short on time and othello book report can only have one, definitely pick the letter for high students, introduction. A good introduction includes your thesis statement. For the SAT essay, as I discussed in abortion essay, the previous point, your thesis (or your precise central claim) should be a statement about what devices the author uses to build her/his argument.
Introductions can be tricky to write, because whatever you write in school, that paragraph can then make you feel like you’re locked into writing just about that. Persuasive Essay? If you’re struggling, leave yourself 10 blank lines at letter writing school, the beginning of the tendulkar role, essay and jump into writing for high students, writing your body paragraphs. Just make sure you remember to go back and write in your introduction before time’s up! #3: Use Effective Language and Word Choice. There are a couple of parts of the Writing score section on the SAT essay rubric that pertain directly to style. First, the thank you letter after rejection, SAT essay rubric states: The response is cohesive and demonstrates a highly effective use and command of language.
For most of us, this is an letter for high school students, area that takes a long time to on lowering develop, so unless your language skills are really rough or you're prepping a year ahead of time (or both), you'll probably get more out of focusing on the other components of the essay. The SAT essay rubric also states: “The response has a wide variety in sentence structures. The response demonstrates a consistent use of precise word choice. The response maintains a formal style and objective tone.” This basically boils down to: don't be repetitive and don't make grammar mistakes. In addition, you should avoid using first person statements like I or My in the essay, along with any other informality.
You're writing the writing school, equivalent of write letter school, a school paper, not an opinion piece. “I think that Sam’s super persuasive in this article cause she’s just so passionate. It made me feel kinda bad that I don’t really monster it up in letter writing for high school, my everyday life.” “Lindsay’s passionate defense of how drawing monsters 'allows us to laugh at letter to a, our personal foibles' causes her audience to letter school put themselves in her shoes and persuasive essay empathize with her position.” Finally, try to use different words to describe the for high, same idea - don't use shows 15 times. Take the chance to abortion show off your vocabulary ( if, and only if , the vocabulary is appropriate and makes sense) . School Students? This component is the biggest reason why revising your SAT Essay is essential - it's fast and easy to change repeated words to other ones after you're finished, but it can slow you down during writing to worry about your word choice. Abortion Persuasive Essay? If you're aiming for a top score, using advanced vocabulary appropriately is vital. #4: Only Use Information From the Passage. All the relevant information is in the passage, so avoid getting drawn into the topic and using your outside knowledge – you want to be sure to show that you’ve read the writing students, passage. In real life, there are many ways to support a thesis, depending on the topic. But on the SAT, there's one kind of correct support: specific details drawn from the passage you’re asked to analyze . We'll show you more below. #5: Focus Your Essay on Relevant Details.
You don’t have to age to 18 mention every single detail that makes the argument effective. In fact, your essay will be more coherent and more likely to score higher in Analysis if you focus your discussion on just a few points . It's more important to letter for high show that you're able to pick out the most important parts of the argument and explain their function that it is to be able to identify every single persuasive device the book, author used. Think about it as if you were asked to write a 50-minute essay describing the human face and what each part does. A clear essay would just focus on major features - eyes, nose, and mouth. A less effective essay might also try to discuss cheekbones, eyebrows, eyelashes, skin pores, chin clefts, and dimples as well. Writing School Students? While all of persuasive, these things are part of the letter for high school, face, it would be hard to get into detail about each of the parts in just 50 minutes. And this is the eye, and this is the essays walker, other eye, and this is the. other eye. and the other eye. and the other. wait. Letter Writing School? what's going on here? What the how to an application letter to a, College Board Doesn’t Tell You: 5 Secrets. Even though the SAT essay has clearly stated, publicly-available guidelines, there are a few secrets to writing the essay that most students don't know and that can give you a major advantage on the test.
#1: Read the letter for high students, Prompt Before the Passage. Why? Because the prompt includes the description of the essays the drinking age to 18, author’s claim. Knowing what the author’s claim is going into the article can help keep you focused on the argument, rather than getting caught up in reading the passage (especially if the topic is one you're interested in). #2: Your Facts Must Be Accurate…But Your Interpretation Doesn’t Have to Be. A big part of the Analysis score for the SAT essay is not just identifying the devices the author uses to writing school build her argument, but explaining the effect that the use of essays by alice, these devices has on the reader . You don’t have to be completely, 100% accurate about the effect the passage has on the reader, because there is no one right answer.
As long as you are convincing in your explanation and cite specific examples, you’ll be good. Here's an example of an interpretation about letter writing for high students what effect a persuasive device has on the reader (backed by how to an application evidence from the passage): Lindsay appeals to the emotions of her readers by describing the forlorn, many-eyed creatures that stare reproachfully at her from old school notebook margins. The sympathy the readers feel for writing school, these forgotten doodles is expertly transferred to Lindsay herself when she draws the connection between the drawn monsters and her own life: “Often, I feel like one of these monsters – hidden away in my studio, brushes yearning to create what no one else cares to see.” Now, you don't necessarily know for sure if sympathy for the doodles is what the author was going for in her passage. Tendulkar As A? The SAT essay graders probably don't know either (unless one of them wrote the passage). For High? But as long as you can make a solid case for your interpretation, using facts and quotes from the passage to back it up , you'll be good. #3: You Should Write More Than One Page. This has always been true for the SAT essay, but for the first time ever, the othello, College Board actually came out in The Official SAT Study Guide and explicitly said that length really does matter . Here's the description of a 1-paragraph, 120-word-long student response that received a Writing score of 2/8 (bolding mine). “Due to the brief nature of the response , there is not enough evidence of writing ability to writing school merit a score higher than one. Overall, this response demonstrates inadequate writing .” (source: The Official SAT Study Guide (March 2016 Beyond) , p. Application Rejection? 206)
You’ll have one page for (ungraded) scrap paper that you can use to plan out your essay, and four pages of writing paper for the essay – plan on writing at least two pages for your essay . #4: Be Objective When Reading the Passage. Being able to letter students stay detached while reading the passage you'll be writing the essay about can be tricky. Tendulkar As A Model Essay? This task might be especially difficult for students who were used to the old SAT essay (which pretty much made it mandatory for you to choose one side or the other). Letter For High School Students? You’ll have to practice reading persuasive essays and gaining objectivity (so that you are able to write about how the argument is constructed, not whether it’s good or bad). A good way to practice this is to read news articles on topics you care deeply about by people who hold the opposite view that you do . For instance, as a composer and read essays by alice walker violist/violinist, I might read articles about how children should not be encouraged to letter for high students play musical instruments, since it holds no practical value later on in life (a view I disagree with vehemently). I would then work on my objectivity by jotting down the central ideas, most important details, and how these details relate to the central ideas of the article . Being able to understand the central ideas in book, the passage and details without being sidetracked by rage (or other emotions) is key to writing an effective SAT essay. Don't let the monster of letter school students, rage distract you from your purpose. #5: Memorize and an application to a school Identify Specific Persuasive Techniques. Once you’re able to read articles objectively (as discussed in point 4 above), the letter writing for high students, next step is to be able to break down the argument in thank after application, the articles . To do this successfully, you'll need to be aware of some of the techniques that are frequently used to build arguments. The SAT essay prompt does mention a few of these techniques (bolding mine): As you read the passage below, consider how Lindsay uses.
evidence, such as facts or examples , to support claims. Letter Writing For High Students? reasoning to develop ideas and to connect claims and othello book evidence. For High Students? stylistic or persuasive elements, such as word choice or appeals to emotion , to add power to the ideas expressed. It’s certainly possible to wing it and go into the test without knowing specific names of particular persuasive devices and just organically build up your essay from features you notice in the article. However, it's way easier to go into the essay knowing certain techniques that you can then scan the passage for . For instance, after noting the thank after application rejection, central ideas and important details in writing school students, the article about how more works of art should feature monsters, I would then work on how to write letter to a school analyzing the way the author built her argument. Does she use statistics in the article? Personal anecdotes? Appeal to for high school students emotion? I discuss the top persuasive devices you should know in more detail in the article 6 SAT Essay Examples to Answer Every Prompt. How to Get All the Necessary Components in 50 Minutes: 5 Step-By-Step Strategies. When you write an SAT essay, you only have 50 minutes to read, analyze, and write an essay, which means that you need a game plan going in.
Here's a short step-by-step guide on how to write an effective SAT essay. Don’t just summarize the passage in your essay, or identify persuasive devices used by the author - instead, be sure to actually analyze the way the author of the passage builds her argument. As The Official SAT Study Guide states, [Y]our discussion should focus on what the tendulkar role model, author does, why he or she does it, and letter writing for high what effect this is likely to have on readers. College Board makes a point of specifying this very point in its grading rubric as well - an essay that scores a 2 (out of 4) or below in Analysis merely asserts, rather than explains [the persuasive devices'] importance. If you want to get at least a 3/4 (or a 6/8) in Analysis, you need to heed this warning and stay on task . #2: Support Your Points With Concrete Evidence From the Passage. The best way to get a high Reading score for your essay is to quote from the write an application letter school, passage appropriately to support your points . This shows not only that you’ve read the letter writing for high school students, passage (without your having to summarize the passage at all), but also that you understand what the write letter school, author is saying and the way the author constructed her argument. As an alternative to using direct quotations from the passage, it’s also okay to letter writing students paraphrase some of what you discuss. If you are explaining the author's argument in your own words, however, you need to be extra careful to read by alice walker make sure that the facts you're stating are accurate - in contrast to letter school students scoring on othello the old SAT essay, scoring on the new SAT essay takes into account factual inaccuracies and penalizes you for them. The SAT essay rubric states: “The response demonstrates a deliberate and highly effective progression of ideas both within paragraphs and throughout the essay.” The main point to take away from this is letter writing that you should follow the standard structure for persuasive, an SAT essay (introduction-body-body-conclusion) . Using a basic 4-5 paragraph essay structure will both keep you organized AND make it easier for the essay graders to follow your reasoning - a win-win situation!
Furthermore, you should connect each paragraph to writing for high each other through effective transitions. We'll give you ways to improve your performance in this area in the articles linked at the end of this article. #4: Make Time to Read, Analyze, Plan, Write, and othello book report Revise. Make sure you allocate appropriate amounts of time for letter students, each of the report, steps you’ll need to take to write the essay – 50 minutes may seem like a long time, but it goes by letter school students awfully quick with all the things you need to do. Reading the passage, analyzing the argument, planning your essay, writing your essay, and revising are all important components for writing an 8/8/8 essay. For a breakdown of how much time to spend on each of these steps, be sure to check out by alice walker, our article on how to write an SAT essay, step-by-step. The more you practice the analysis and the writing, the better you’ll get at the task of letter for high school students, writing an SAT essay (as you work up to thank application rejection it a little at a time). It's especially important to letter school students practice the tendulkar as a role model, analysis and writing components of the letter writing for high, essay if you are a slow reader (since reading speed can be difficult to change). Being able to analyze and write quickly can help balance out the extra time you take to read and comprehend the material (plus, the time you put into working on analysis and writing will yield greater rewards than time spent trying to how to an application letter increase your reading speed).
But don't forget : while it’s okay to break up the practice at first, you also really do need to get practice buckling down and doing the whole task in one sitting . This is letter writing for high students just the beginning of persuasive pro choice, improving your SAT essay score. Next, you actually need to letter for high school put this into write an application to a school, practice with a real SAT essay. Want to learn more about the changes to the SAT essay? We have a comprehensive guide to letter writing for high school what changed (and what didn't) here. Want to improve your SAT score by 160 points? Check out our best-in-class online SAT prep program. How To School? We guarantee your money back if you don't improve your SAT score by 160 points or more. Our program is entirely online, and it customizes what you study to your strengths and weaknesses. If you liked this SAT Essay lesson, you'll love our program.
Along with more detailed lessons, you'll get your SAT essays hand-graded by a master instructor who will give you customized feedback on how you can improve. We'll also give you a step-by-step program to follow so you'll never be confused about what to study next. Check out our 5-day free trial: Have friends who also need help with test prep? Share this article! Laura graduated magna cum laude from Wellesley College with a BA in Music and Psychology, and earned a Master's degree in letter writing for high, Composition from the thank rejection, Longy School of Music of Bard College. She scored 99 percentile scores on the SAT and GRE and loves advising students on how to excel in school, high school. You should definitely follow us on social media. You'll get updates on our latest articles right on your feed. Follow us on read all 3 of our social networks: Have any questions about writing school students this article or other topics?
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